Chemical Engineering: Blue-Bottle Control

E6.3.3.2

The blue bottle experiment is a model experiment for a organic redox system. The redox indicator methylene blue is reduced by glucose to its colourless form which is then oxidised by air to form again the coloured version. The air is led into solution by a pump. Additional glucose in solution will then reduce it to its colourless form. The process is reversible. In this experiment a automated version with a two-point control system is used.

Topics

To learn about organic redox reactions and the redox indicator methylene blue
To perform the blue bottle experiment in a new, automated version
To set up and use a two - point control system
To perform photometry using an immersion photometer
To use carbohydrates such as glucose as a reducing age

1	524 013 8	Sensor-CASSY 2
1	524 220 4	CASSY Lab 2
1	524 069 4	Immersion photometer S
1	666 425 4	Panel frame C50, two-level, for CPS
1	666 438	Woulff's bottle with manometer, CPS
1	667 3095 4	Screw cap, GL 45, with hole
1	667 3107 4	Silicone seal GL 45/26, 10 pcs
1	666 482	Aeration pump, controllable, CPS
1	501 44 4	Connecting leads, 19 A, 25 cm, red/blue, pair
1	667 7977 8	Electronic Balance 440-3N, 200 g : 0.01 g
2	664 157 4	Watch glass dish 125 mm Ø
2	666 966 4	Spoon-ended spatula, PP, 180 mm
1	666 003 4	Pipetting ball (Peleus ball)
1	665 996 4	Graduated pipette, 5 ml
1	665 756 4	Measuring cylinder 500 ml, with plastic base
1	604 501 4	PVC tubing, 7 mm diam., 1 m
1	604 460 4	Hose clamp, 8...12 mm
1	602 347 4	Laboratory bottle, 500 ml, GL 45 thread
1	673 2920 4	Methylene blue solution, 100 ml Warning H226 H319
1	673 6800 4	Sodium hydroxide, pellets, 100 g Danger H314 H290
1	672 1100 4	D(+)-Glucose, 100 g
1	675 3400 4	Water, pure, 1 l
1		additionally required: 1 PC with Windows 7 / 8.1 / 10 (x86 or x64)

Chemical Engineering: Blue-Bottle Control

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